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Bioss α sma
GDL treatment improves liver function and fibrosis-related serological indices in WD model rats. A Serum levels of hyaluronic acid (HA), laminin (LN), type III pro-collagen (PC-III), collagen IV (C-IV), aspartate aminotransferase (ALT), and alanine aminotransferase (AST) in each group ( n = 6). B Hepatic copper content across different groups ( n = 6). C Protein expression <t>of</t> <t>α-SMA</t> and collagen I in rat liver tissues, as determined by Western blotting ( n = 3). D Protein expression <t>of</t> <t>α-SMA</t> and collagen I in LX-2 cells treated with CuSO₄ and GDL-containing serum ( n = 3). GAPDH was used as a loading control. Data are presented as means ± SD. Statistical significance was determined by one-way ANOVA with Bonferroni’s post hoc test. ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. model group; * p < 0.05, ** p < 0.01 vs. CuSO 4 group
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GDL treatment improves liver function and fibrosis-related serological indices in WD model rats. A Serum levels of hyaluronic acid (HA), laminin (LN), type III pro-collagen (PC-III), collagen IV (C-IV), aspartate aminotransferase (ALT), and alanine aminotransferase (AST) in each group ( n = 6). B Hepatic copper content across different groups ( n = 6). C Protein expression of α-SMA and collagen I in rat liver tissues, as determined by Western blotting ( n = 3). D Protein expression of α-SMA and collagen I in LX-2 cells treated with CuSO₄ and GDL-containing serum ( n = 3). GAPDH was used as a loading control. Data are presented as means ± SD. Statistical significance was determined by one-way ANOVA with Bonferroni’s post hoc test. ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. model group; * p < 0.05, ** p < 0.01 vs. CuSO 4 group

Journal: 3 Biotech

Article Title: Gandouling protects against hepatic fibrosis in Wilson disease through the lncRNA-SNHG7/miR-29b/DNMT3A pathway

doi: 10.1007/s13205-026-04769-0

Figure Lengend Snippet: GDL treatment improves liver function and fibrosis-related serological indices in WD model rats. A Serum levels of hyaluronic acid (HA), laminin (LN), type III pro-collagen (PC-III), collagen IV (C-IV), aspartate aminotransferase (ALT), and alanine aminotransferase (AST) in each group ( n = 6). B Hepatic copper content across different groups ( n = 6). C Protein expression of α-SMA and collagen I in rat liver tissues, as determined by Western blotting ( n = 3). D Protein expression of α-SMA and collagen I in LX-2 cells treated with CuSO₄ and GDL-containing serum ( n = 3). GAPDH was used as a loading control. Data are presented as means ± SD. Statistical significance was determined by one-way ANOVA with Bonferroni’s post hoc test. ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. model group; * p < 0.05, ** p < 0.01 vs. CuSO 4 group

Article Snippet: The membranes were then incubated overnight with antibodies against DNMT3A (Bioss, Item No. bs-23029R, 1:2000, Rabbit, 1:1000, Rabbit), p-DNMT3A (Bioss, Item No. bs-14399R, 1:2000, Rabbit), α-SMA (Bioss, Item No. Bsm-33187 M, 1:2000, Mouse; No. bs-0189R, 1:1000, Rabbit), Collagen I (Bioss, Item No. bs-7158R, 1:1000, Rabbit; No. AB260043 , 1:1000, Rabbit), Beclin-1 (Abcam, Item No. ab210498, 1:2000, Rabbit; No. ab62472, 1:1000, Rabbit), LC3B (CST, Item No. 3868s, 1:1000, Rabbit; No. 43566 S, 1:1000, Rabbit), and p62 (Bioss, Item No. bs-2951R, 1:1000, Rabbit) at 4°C with gentle shaking.

Techniques: Expressing, Western Blot, Control